Romanowsky staining using liquid-based cytology: A pilot study using Cytolyt®/HESPANDER® processing solution for ThinPrep® preparations

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Abstract

Background:

Liquid-based cytology (LBC) has been used to prepare and examine many types of samples. However, the use of Romanowsky stains for LBC has not yet been evaluated. Herein, we report a technique for the use of the Romanowsky May–Grünwald–Giemsa (MGG) stain using a ThinPrep® preparation technique (MGG-LBC).

Methods:

KPL-1 breast cancer cells at a density of 1.25 × 105/20 ml were compared in conventional smear and MGG-LBC preparations. Cell size, nucleus/cytoplasm (N/C) ratio, and morphological findings were investigated. Clinical samples including voided urine and pleural effusion were also examined in MGG-LBC preparations.

Results:

Cellularity appeared lower with MGG-LBC compared with Papanicolaou-stained smears using the ThinPrep® method, though the cell size and N/C ratio showed similar tendencies. Reactive mesothelial cells, normal urothelial cells, urothelial carcinoma cells, crystals, and bacteria were all clear enough for diagnostic purposes after MGG-LBC.

Conclusion:

Romanowsky staining is necessary for the cytodiagnosis of some conditions. MGG-LBC may contribute to the cytodiagnostic results using LBC preparations. Diagn. Cytopathol. 2015;43:960–965. © 2015 Wiley Periodicals, Inc.

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