1Laboratory of Experimental Neuropathology, Instituto Nacional de Neurología y Neurocirugía, Cuidad de México, Mexico2Service of Gynecology, Servicio de salud del Estado de Guerrero, Germany3Deutschen Krebsforschungszentrum, Heidelberg, Germany4Translational Research and Cell Therapy Laboratory, Metabolic Research Unit, National Medical Center “Siglo XXI” IMSS, Mexico City, Mexico
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We performed cytological examination of urethral brushing to study aberrant E-Cadherin expression as a possible marker for papillomavirus in cytological samples.A total of 30 cytobrush male urethral smears were examined E-cadherin expression and human papillomavirus (hpv) was confirmed using PCR-DNA.The age range was 19–36 years (mean: 27 years), 17 (56.7%) cases corresponded to low-risk HPV and 13 (43.3%) cases were high-risk HPV. The mean age ranges were 25.77 ± 5.90 years for high-risk HPV and 26.77 ± 4.31years for low-risk HPV.Pap smears showed dyskeratosis in 23 (76.7%) cases, koilocytes in 13 (43.3%) cases, infection or bacterial background in 14 (43.7%) cases, suggestive changes of Gardnerella infection in 7 (23.3%) cases, and Chlamydia in 3 (10%) cases.Immunohistochemistry was positive for membrane E-Cadherin; there was weak expression in 13 (43.3%) cases, moderate expression in 11 (36.7%) cases (P = 0.109) (Figs. 3a and b), and strong expression in 6 (20%) cases. There were a statistically significant correlations between E-Cadherin expression and koilocytes (P = 0.007), individual cell dyskeratosis (P = 0.041), and HPV risk (P = 0.000).We concluded that the loss of E-Cadherin membrane expression was greater in high-risk HPV cases and was associated with individual cell dyskeratosis features and koilocytes. There were statistically significant correlations between E-Cadherin expression and dyskeratosis (P = 0.043), koilocytes (P = 0.007), and type of HPV (P = 0.000). Using male urethral smears to test for the loss of E-Cadherin membrane expression is simple, rapid, specific and more sensitive than conventional morphologic observations. We concluded that E-Cadherin can be used to discriminate between high- and low-risk papillomavirus in urethral cytologic specimens. Diagn.