A highly sensitive fluorescent indicator dye for calcium imaging of neural activityin vitroandin vivo

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Abstract

Calcium imaging of individual neurons is widely used for monitoring their activityin vitroandin vivo. Synthetic fluorescent calcium indicator dyes are commonly used, but the resulting calcium signals sometimes suffer from a low signal-to-noise ratio (SNR). Therefore, it is difficult to detect signals caused by single action potentials (APs) particularly from neuronsin vivo. Here we showed that a recently developed calcium indicator dye, Cal-520, is sufficiently sensitive to reliably detect single APs bothin vitroandin vivo. In neocortical neurons, calcium signals were linearly correlated with the number of APs, and the SNR was > 6 forin vitroslice preparations and > 1.6 forin vivoanesthetised mice. In cerebellar Purkinje cells, dendritic calcium transients evoked by climbing fiber inputs were clearly observed in anesthetised mice with a high SNR and fast decay time. These characteristics of Cal-520 are a great advantage over those of Oregon Green BAPTA-1, the most commonly used calcium indicator dye, for monitoring the activity of individual neurons bothin vitroandin vivo.

Calcium imaging of individual neurons is widely used for monitoring their activityin vitroandin vivo. Synthetic fluorescent calcium indicator dyes are commonly used, but the resulting calcium signals sometimes suffer from a low signal-to-noise ratio (SNR).

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