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The aim of this study was to evaluate and compare the surface tension of different liquids that are used as potential agents to mix with calcium hydroxide in root canal treatment. We also tried to evaluate final surface tension values of the calcium hydroxide mixed with glycerin, Ringer's solution, anesthetic solution, and saline. Surface tension values were measured using a “Du Noüy” tensiometer. The results showed statistically significant differences between vehicles alone and the calcium hydroxide combinations and also indicated that anesthetic solution (p = 0.018, p ≤ 0.02) is the most favorable vehicle with the lowest surface tension values.