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Purpose: Assessment of patients with mitral valve stenosis (MS) requires a detailed evaluation of mitral valve anatomy, mitral valve area and presence concomitant valve diseases. Our aim was to evaluate planimetric mitral valve (MV) area, mitral leaflet seperation index (MVSI) and transmitral flow velocities using transthoracic echocardiography (TTE) in patients with isolated MS and to compare these measurements with cardiac magnetic resonance imaging (CMRI).Methods: Thirty-one patients (mean age 50.4±10.2, 90.3% women) with isolated MS who were in normal sinus rhytm were included in the study. Patients with ejection fraction <50%, atrial fibrillation, moderate-to severe mitral valve insufficiency, moderate-to severe stenosis and insufficiency in other valves and previous commissurotomy and valvulotomy were excluded. Planimetric MV area, MVSI, diastolic velocities were measured with TTE and CMRI.Results: There was a strong correlation between measurements of planimetric MV area, MVSI, transmitral diastolic velocities (p<0,0001). When patients were divided into groups as mild MS and moderate-to severe MS, correlation between the methods was persisted. CMRI's sensitivity, specifity, negative and positive predictive values were found 84.2%, 100%, 80%, 100% in patients with MV area<1.5 cm2 in TTE measurements.Conclusions: We suggest CMRI is a complementary method in patients with MS for diagnosis and follow-up especially when echocardiographic data was insufficient or there was discrepancy between clinic of the patient and catheterization findings.