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Purpose: Recently vender independent speckle analysis software has become available (TomTec, Germany). We aim to compare two commercially available 3D speckle tracking software, one vender dependent (Toshiba Medical Systems) and one vender independent (TomTec, Germany) using cardiac magnetic resonance imaging as a reference.Methods: 21 volunteers underwent 3D echocardiography on a Phillips iE33 (X3-1 probe / 3MHz) and Toshiba 4D Artida (3MHz/PST-30SBT probe) and CMR on the same day. The same transducer frequency and similar volume rates were used.Toshiba 3D speckle tracking software was used to analyse images obtained from the Toshiba system and TomTec software was used to analyse images obtained from both Philips and Toshiba systems.Results: Agreement between echo and CMR were excellent for longitudinal strain (mean difference 0.6-1.6%); good for circumferential strain (mean difference 1 -3.71%) and poor for radial strain (mean difference 12.81-25.32%).Interobserver echocardiography variability showed agreement was excellent for longitudinal strain (mean difference 0.1 – 1.6%); good for circumferential strain (mean difference 1.36- 6.2%) and modest for radial strain (mean difference 7.55-13.5%).Analysis of Toshiba datasets on both Toshiba 3D WMT and TomTec software showed agreement was excellent for longitudinal strain (BAA mean difference 1%), good for circumferential strain (BAA mean difference 3.6%) and poor for radial strain (BAA mean difference 15.5%).Conclusions: Vender independent and dependent 3D speckle tracking echocardiography software shows good levels of agreement when compared with CMR for the measurement of longitudinal and circumferential strain. However, the high variability for radial strain assessment is a concern and further work on speckle tracking algorithms may be needed to identify the potential cause for this.