Megakaryocyte development is normal in mice with targeted disruption ofTescalcin

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Abstract

Background

Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H + exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34 + hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation.

Objective

To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis.

Methods

We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development.

Results

Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal.

Conclusion

Tescalcin does not appear to be necessary for normal megakaryocyte development.

Highlights

▸ Upregulation of tescalcin gene augments megakaryocyte development in K562 and HEL cells. ▸ Mice with targeted disruption of tescalcin gene were created. ▸ Platelet and megakaryocyte numbers are normal in tescalcin-knockout mice. ▸ Polyploidization and Fli-1 expression in megakaryocytes of tescalcin-knockout mice is normal. ▸ Tescalcin does not have an essential role in megakaryocyte differentiation in a murine model.

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