Chronological aging of budding yeast cells results in a reduction in subsequent replicative life span through unknown mechanisms. Here we show that dietary restriction during chronological aging delays the reduction in subsequent replicative life span up to at least 23 days of chronological age. We further show that among the viable portion of the control population aged 26 days, individual cells with the lowest mitochondrial membrane potential have the longest subsequent replicative lifespan. These observations demonstrate that dietary restriction modulates a common molecular mechanism linking chronological and replicative aging in yeast and indicate a critical role for mitochondrial function in this process.Highlights
▸ DR delays the reduction in replicative lifespan associated with chronological age. ▸ MMP during chronological aging predicts subsequent replicative lifespan. ▸ Chronologically aged cells with highest MMP cannot complete a cell division. ▸ Chronologically aged cells with lowest MMP maintain a full replicative lifespan.