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The purpose of this study was to measure the shape of the anterior and posterior surface of human cadaver lenses in situ using a corneal topography system. Measurements were performed on 13 pairs of eyes using the PAR Corneal Topography System (PAR-CTS). The age of the donors ranged from 46 to 93 years, with an average age of 76·4 years. Anterior lens topography was measured after excision of the cornea and iris. Posterior lens topography was measured after sectioning the posterior segment and adherent vitreous. The PAR-CTS files providing raw surface height were exported for analysis. In each surface, 18 meridians separated by 10° were fitted using conic sections to obtain values of the apical radius of curvature (R) and shape factor (p). The average apical radius of curvature and asphericity were R=10·15±1·39 mm and p=4·27±1·39 for the anterior surface and R=−6·25±0·79 mm and p=−0·64±1·85 for the posterior surface. A significant variation of the radius of curvature and shape factor as a function of the meridian angle (lens astigmatism) was found in some lenses. Contrary to previous findings, the anterior lens surface was found to steepen toward the periphery.