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The purpose of this study is to investigate if a readily available cell line (APRE-19) may be used to study in vitro function of visual cycle enzymes such as lecithin:retinol acyltransferase (LRAT). Cells incubated with exogenous retinol accumulated intracellular all-trans retinol and all-trans retinyl ester. Membrane proteins from ARPE-19 exhibited LRAT activity, which was inhibited by an LRAT inhibitor, retinyl bromoacetate (RBA). Gene microarray and Western blot results indicated that ARPE-19 cells expressed LRAT transcript and the LRAT protein. Therefore, our data show that ARPE-19 contains an active LRAT enzyme and suggest that it is an appropriate cell system to study visual cycle enzymes.