PD-loop technology: PNA openers at work

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A concise survey of the emerging PD-loop technology is presented, which outlines several exemplary methods with robust DNA diagnostic potential: duplex DNA capture, topological DNA labeling, nondenaturing DNA sequencing and hybridization of molecular beacons to double-stranded DNA. Advantages of these new PNA-based assays over existing techniques for sequence-specific detection and manipulation of DNA duplexes are discussed. Future prospects for the further development of PD-loop technology are highlighted.Dedicated to my former mentors and now colleagues, Peter E Nielsen and Maxim D Frank-Kamenetskii, on occasion of their 50th and 60th anniversaries, respectively and to 10 years of PNA, which is the key element in PD-loop technology.

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