|| Checking for direct PDF access through Ovid
The adult human gut is a complicated ecosystem in which host–bacterium symbiosis plays an important role. Bacteroides thetaiotaomicron is a predominant member of the gut microflora, providing the human digestive tract with a large number of glycolytic enzymes. Expression of many of these enzymes appears to be controlled by histidine kinase receptors that are fused into unusual hybrid two-component systems that share homologous periplasmic sensor domains. These sensor domains belong to the third most populated (HK3) family based on a previous unpublished bioinformatics analysis of predicted histidine kinase sensors. Here, we present the crystal structures of two sensor domains representative of the HK3 family. Each sensor is folded into three domains: two-seven-bladed β-propeller domains and one β-sandwich domain. Both sensors form dimers in crystals, and one sensor appears to be physiologically relevant. The folding characteristics in the individual domains, the domain organization, and the oligomeric architecture are all unique to HK3 sensors. Sequence analysis of the HK3 sensors indicates that these sensor domains are shared among other signaling molecules, implying combinatorial molecular evolution.The structural data for the crystallographic results for HK3 BT4673S and HK3 BT3049S have been deposited in the Protein Data Bank under accession numbers 3OTT and 3V9F, respectively.HK3BT3049S and HK3BT3049S bind by x-ray crystallography (View interaction)HK3BT3049S and HK3BT3049S bind by molecular sieving (View interaction)HK3BT3049S and HK3BT3049S bind by cosedimentation through density gradient (View interaction)HK3BT4673s and HK3BT4673s bind by cosedimentation through density gradient (View interaction)HK3BT4673s and HK3BT4673s bind by molecular sieving (View interaction)The human gut microbiome includes bacteria that symbiotically process sugar foodstuffs under control of diverse histidine kinase receptors. Sensor domains from these receptors, which are thought to detect available carbohydrates, belong to the third most populated (HK3) sensor family. This paper describes crystal structures for two HK3-family sensors. A dimer has β-propeller and β-sandwich domains disposed with apparent physiological relevance.