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Methionine adenosyltransferase (MAT) is a family of enzymes that utilizes ATP and methionine to produce S-adenosylmethionine (AdoMet), the most crucial methyl donor in the biological methylation of biomolecules and bioactive natural products. Here, we report that the MAT from Sulfolobus solfataricus (sMAT), an enzyme from a poorly explored class of the MAT family, has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP. To investigate the molecular basis for AdoMet analog production, we have crystallized the sMAT in the AdoMet bound, S-adenosylethionine (AdoEth) bound and unbound forms. Notably, among these structures, the AdoEth bound form offers the first MAT structure containing a non-native product, and cumulatively these structures add new structural insight into the MAT family and allow for detailed active site comparison with its homologs in Escherichia coli and human. As a thermostable MAT structure from archaea, the structures herein also provide a basis for future engineering to potentially broaden AdoMet analog production as reagents for methyltransferase-catalyzed ‘alkylrandomization’ and/or the study of methylation in the context of biological processes.PDB IDs: 4HPV, 4L7I, 4K0B and 4L2Z.EC 220.127.116.11• sMAT and sMAT bind by x-ray crystallography (View interaction)We report crystal structures of MAT from Sulfolobus solfataricus, an archaeal enzyme that can produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP. Notably, the AdoEth-bound structure offers the first MAT form containing a non-native product and cumulatively, the structures herein provide as a basis for future engineering to broaden AdoMet analog production as reagents for methyltransferase-catalyzed ‘alkylrandomization’.