Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile

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Abstract

Aim:

The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile.

Material & methods:

DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR.

Results:

Global DNA Methylation Index (p = 0.02) and promoter methylation ofSSBP2(p = 0.01) andESR1(p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation ofAPC,CDKN2A,ESR1,PGP9.5andSSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%.

Conclusion:

Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.

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