Development of a Dot-blot System to Detect Gluten in Food


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Abstract

We have designed a quick, easy, and reliable enzyme immunoassay system based on dot-blot methodology, capable of determining the presence of gluten or its derivatives in food. We also describe a new solution to extract the prolamin from flours. The sensitivity of the system is less than 5 ppm using a maximum volume of 2 μl.

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