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Male-to-female gender reassignment surgery may lead to unsatisfactory vaginal length. No standard approaches are established to treat these patients. We present a case of vaginoplasty using the laparoscopic Davydov technique performed on a male-to-female transgender patient. Our case provides a novel approach to treating this rare condition and introduces Davydov procedure as a potentially effective and safe treatment. Preoperative endovaginal and endoanal 3-dimensional ultrasounds and a pelvic magnetic resonance imaging of the case are presented to provide details of this patient's unique anatomy.