Effects of Age and Long-Term Caloric Restriction on the Aqueous Collecting Channel in the Mouse Eye

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SummaryPurposeThe mouse aqueous collecting channel, part of the mouse aqueous outflow pathway, was measured using a computer-assisted image analysis system. We used ad libitum—fed and calorie-restricted mice to investigate the effects of age and long-term caloric restriction on the lumen size of the channel.ResultsThe ad libitum-fed mice showed a significant age-related reduction in the lumen area. In the ad libitum-fed group, the lumen area of the channel decreased by 30% at 30–35 months of age (p < 0.01), and the anteroposterior width declined by 21% (p < 0.001) as compared with mice 3–5 months of age. The calorie-restricted mice did not display any reduction with age in the lumen area or the anteroposterior width of the channel. When compared with the age-matched calorie-restricted mice, the lumen area of the channel of the ad libitum-fed mice measured less by 34%, and anteroposterior width less by 21% at 30–35 months of age (p < 0.01).ConclusionsThese results indicate that an age-related morphological alteration in the mouse aqueous collecting channel occurred and that it is delayed by long-term caloric restriction. This suggests a possible role of life-long caloric restriction in reducing glaucomatous damages and perhaps delaying glaucoma development.

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