158 Strategies to Improve Haemostasis in Trauma: Evaluation of Thrombosomes® in the Presence of Native Platelet Dysfunction

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Abstract

The ability of Thrombosomes®, a lyophilised human platelet product, to enhance thrombus formation in platelet free plasma has been previously demonstrated in the Badimon chamber, an ex-vivo model of deep arterial injury. Using this model, the study evaluates their contribution to thrombus formation in the presence and absence of native platelet dysfunction.

Aspirin and clopidogrel were administered to twelve healthy participants to render native platelets dysfunctional. Blood was drawn from an antecubital vein into the Badimon chamber. High and low shear rheological conditions were simulated in separate chambers each containing a thrombogenic substrate and Thrombosomes® were added at three different concentrations; 0, 50 and 200 × 106/mL.

In the presence of native platelet dysfunction and under high shear stress a concentration of 50 × 106/mL was found to contribute to clot formation (p < 0.05). The effect was not replicated under either condition, in the presence or absence of native platelet dysfunction at 0 and 200 × 10 Thrombosomes®/mL.

In conclusion, at a low concentration in an ex-vivo model Thrombosomes® enhance thrombus formation in the presence of dysfunctional native platelets. This lends further support to their potential use as a haemostatic agent in the management of trauma-related injuries.

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