181 Elucidating The Role Of β3-receptors In Perivascular Adipose Tissue

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Abstract

Introduction

Perivascular adipose tissue (PVAT) has been shown by various studies to have an anti-contractile effect on the arteries it surrounds. The mechanism by which PVAT exerts its anti-contractile effect is not well understood, and is likely to involve the release of PVAT-derived relaxing factors (PVRF) such as adiponectin, that causes hyperpolarisation and relaxation of the vascular smooth muscle. The PVAT anti-contractile effect is lost in obesity, therefore understanding this mechanism is vital for creating novel treatments of obesity-related hypertension. We propose that the mechanism involves stimulation of adipocyte β3-adrenoceptors in PVAT.

Methods

The contractile responses of noradrenaline and serotonin were assessed in rat mesenteric small arteries using wire myography with and without PVAT, and the effects of β3-agonist CL-316,243 (10µM), β3-antagonist SR59230A (1 µM), and adiponectin type-1 receptor blocking peptide were studied (2.5µM).

Results

PVAT was shown to have a significant anti-contractile effect on the responses to both noradrenaline (n = 5, P = 0.0135) and serotonin (n = 5, P = 0.0096). The CL-316,243 was found to have no significant effect on either the concentration-response to noradrenaline (n = 6, P = 0.5584) or serotonin (n = 4, P = 0.2072). SR59230A induced a significant reduction in the anti-contractility of PVAT in vessels stimulated with the highest concentration of noradrenaline (n = 3, P = 0.0018), but not serotonin (n = 3, P = 00.0621). Adiponectin receptor type-1 blocking peptide has been shown to abolish the noradrenaline-induced anti-contractile effect; however in this study the blocking peptide had no significant effect on the serotonin-induced PVAT anti-contractility (n = 4, P = 0.505).

Conclusions and Implications

These results suggest that β3-adrenoceptors may be involved in noradrenaline PVAT anti-contractility, but the serotonin-induced anti-contractile effect appears to be independent of events at β3-adrenoceptors, and involves a different PVRF.

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