Atrial fibrillation is a kind of most common arrhythmia in the adult population. The activated renin-angiotensin-aldosterone system (RAS) has been reported to play an important role in the pathogenesis of atrial fibrillation (AF). The aim of this study was to investigate the relation between nonfamilialAF and polymorphisms in RAS.Methods
A total of 701 patients with nonfamilial AF and 763 healthy subjects or patients only with hypertension as controls were selected. Some polymorphisms in the angiotensin I-converting enzyme (ACE) gene (rs8066114), angiotensinogen (AGT) gene (rs7539020, rs3789678, rs2478544, rs11568023, rs2478523, rs4762, rs699) and aldosterone synthase (CYP11B2) gene (rs3802230, rs3097) were genotyped. Single-locus analysis, multilocus haplotype analysis and multifactor-dimensionality reduction method were used in this study.Results
In single-locus analysis, we found rs2478523 and rs11568023 in AGT gene were associated with nonfamilial AF in Chinese Han population. Frequencies of the rs2478523-TT and rs11568023-TT genotype were significantly higher in cases than in controls (p = 0.033 and 0.003, respectively). There was no linkage disequilibrium between rs2478523 and rs11568023 in AGT gene. In multilocus haplotype analysis, the angiotensinogen gene haplotype and CYP11B2 gene haplotype profile were not significantly different between cases and controls. Furthermore, significant gene-gene interactions were detected by the multifactor-dimensionality reduction method. Gene-to-gene interaction was not foundto be relatedto AF.Conclusions
Via a large-scale case-control study, we found rs2478523 and rs11568023 in AGT were associated with nonfamilial AF in Chinese Han population. These two sites are potential susceptible loci of AF.