ASSA14-14-07 Expressions of endothelin-converting enzyme in primary cultured atrial myocytes after rapid electrical-field stimulation

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Abstract

Background

To study the expressions of endothelin-converting enzyme (ECE2) at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model.

Methods

Primary rat atrial myocytes were cultured and a rapid paced cell model was established. The atrial cells were divided into five groups with pacing durations within 0 to 24 h. The polymerase chain reaction (RT-PCR) and Western blot (WB) were applied to detect the messenger ribonucleic acid (mRNA) and proteins expression of ECE2.

Results

mRNA expression of ECE2 increased after 3 h of rapid pacing and continued to elevate in a time dependent manner. Similarly, changes of ECE2 protein were paralleled with mRNA expression.

Conclusion

Expressions of ECE2 increased in early phase of rapid paced atrial myocytes. It implicated the ECE2 and endothlin-1 was implicated in pathological remodelling of atrial fibrillation.

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