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To study the expressions of endothelin-converting enzyme (ECE2) at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model.Primary rat atrial myocytes were cultured and a rapid paced cell model was established. The atrial cells were divided into five groups with pacing durations within 0 to 24 h. The polymerase chain reaction (RT-PCR) and Western blot (WB) were applied to detect the messenger ribonucleic acid (mRNA) and proteins expression of ECE2.mRNA expression of ECE2 increased after 3 h of rapid pacing and continued to elevate in a time dependent manner. Similarly, changes of ECE2 protein were paralleled with mRNA expression.Expressions of ECE2 increased in early phase of rapid paced atrial myocytes. It implicated the ECE2 and endothlin-1 was implicated in pathological remodelling of atrial fibrillation.