Investigation of tissue preparation conditions for non-radioactive : localization of transforming growth factor-α message in rat kidneyin situ: localization of transforming growth factor-α message in rat kidney hybridization: localization of transforming growth factor-α message in rat kidney

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Abstract

Summary

A non-radioactive method of in situhybridization was used to localize transforming growth factor-α mRNA in epithelial cells of collecting ducts and tubules in rat kidney tissue sections. The intensity and specificity of staining were assessed under a variety of tissue preparation conditions, including a direct comparison of paraffin against frozen sections. Under optimal conditions, both the signal strength and the cellular localization of the growth factor message were superior in paraffin sections. The staining method could also be used to localize the message in lung tissue, indicating that the procedure is generally applicable to other tissues. Our results indicate that the use of paraffin sections for non-radioactive in situ hybridization affords a number of advantages for the localization of specific messages in tissue sections.

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