The abundance, biomass, and life history traits of Mysis relicta were evaluated in the spring, summer, and autumn of 2000 at 8 nearshore (45 m) and 8 offshore (75-110 m) stations throughout central and southern Lake Michigan. Abundance was also evaluated on a smaller scale during June 2000 at 4 nearshore and 4 offshore stations in southeastern Lake Michigan. For large-scale sampling, the abundance of M. relicta did not differ among locations in the spring. In the summer and autumn the abundance of M. relicta was similar among offshore stations with the exception of one station each season; for nearshore stations, abundance was generally highest off Pentwater, Michigan. The abundance of mysids was not consistently high for central or southern basin sites, although overall biomass was higher in the southern basin each season. Abundance of Mysis was positively correlated with bottom depth, but not with bottom water temperature, surface water temperature, or mean chlorophyll concentration. Within the smaller region in southeast Lake Michigan, the abundance of M. relicta differed among locations for both nearshore and offshore stations. Brood size and size of reproductive females did not differ among lake wide locations, but the proportion of females with broods and the size distribution of M. relicta did.