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To examine the feasibility of using ecophysiological data from excised leaves for a meteorological simulation model of gas exchange, we compared the obtained gas exchange rates and the modelled ones using excised leaves and those using intact leaves. Instantaneous gas exchange rates of excised leaves and stomatal control in response to environmental conditions were not significantly different from those of attached leaves. Modelled gas exchange rates based on excised leaf data showed a good fit to the diurnal patterns of in situ measurements. This suggests that use of excised leaf data to predict gas exchange of intact leaves is permissible as long as the effects of excision are pre-screened as described in this paper. Copyright © 2011 John Wiley & Sons, Ltd.