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The genomic loci of four distinct phospholipase C genes (PLC-β, PLC-γI, PLC-δ and PLC-γII) were examined for restriction fragment length polymorphisms (RFLPs) between the genomes of three normotensive [Sprague-Dawley, Donryu and Wistar—Kyoto (WKY)] and two closely related hypertensive [spontaneously hypertensive (SHR) and SHR stroke-prone (SHR-SP)] rat strains. The RFLPs observed between SHR and WKY were classified into three types. Type I RFLPs are those observed at 4.3 kilobase (kb) and 1.9kb by Aval digestion for PLC-γ probe and at 1.9kb by Accl digestion for PLC-β probe, where RFLP banding patterns are conserved in two hypertensive (SHR and SHR-SP) and one normotensive (Sprague-Dawley) strains. Type II RFLPs are those observed by Accl, BamHI, EcoRl and Psti digestions for PLC-β probe, where RFLP pattern observed in SHR is shared by one normotensive (Sprague-Dawley) strain but not by SHR-SP, WKY or Donryu rats. Type III RFLPs are those detected at 6.3 kb band by Bg/ll digestion for PLC-β probe and at 1.0 kb by BamHI digestion for PLC-γII probe, where RFLP pattern observed in SHR is shared by two normotensive rats other than WKY. No RFLP was found for PLC-γI probe after testing 13 restriction enzymes. Since PLC plays a pivotal role in regulating the intracellular calcium concentration and the intracellular signal transduction, these RFLPs may offer a valuable tool for the analysis of genomic predisposition for hypertension. Furthermore, our result suggesting the presence of strain-related polymorphism indicates that a comparison of polymorphism of any genes between SHR and WKY only is not sufficient to bring about a definite conclusion concerning the relationship between the gene and the aetiology of hypertension.