P-280 Serum MIF and TNF- α Level in Patients with Ulcerative Colitis

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Abstract

Background:

UC is an inflammatory disease of the colonic mucosa and seems to result from a complex series of interactions. Cytokine productions are under genetic control and are involved in regulation of immune and inflammatory response. Macrophage migration inhibitory factor is an important pro inflammatory cytokine and plays a critical role in immune and inflammatory responses. TNF- α involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types. Plasma MIF and TNF- α was reported to elevated in patients with UC and CD compared with healthy controls. MIF levels may be related to the pathogenesis and induction of inflammatory cytokines in UC.

Background:

Aim of the present study was to evaluate the serum concentration of MIF and TNF- α in patients with Ulcerative colitis, Irritable Bowel Syndrome (IBS) and healthy control.

Methods:

A total of 247 subjects (59 UC, 127 IBS and 61 Healthy controls) were enrolled for the study fulfilling inclusion and exclusion criteria. Blood samples were obtained after informed consent for serum separation. Enzyme linked immune sorbent assay (ELISA) was performed using commercial Laboratory kit for human MIF and TNF- α according to manufacturers instruction. A P value of less than 0.05 was considered significant. Statistical analysis was conducted by using SPSS 16.0 (SPSS, Chicago).

Results:

MIF level in UC and IBS patients was found to be significantly high (P < 0.0003) having mean SD 3.55 ± 1.3 and (P < 0.0006) having mean SD 3.96 ± 1.39 (Z score = 3.19) respectively when compared to healthy control. When TNF- α level was compared in UC and IBS with healthy control in both cases significant correlation was found having P < 0.001 (Z score = 8.014 and 8.710 respectively). When UC was compared with IBS no significant correlation was found in both MIF and TNF- α.

Conclusions:

High level of MIF and TNF- α in serum of patients with UC and IBS supports that more than one cytokine may play role in inflammation and they could be an effective factor in the pathogenesis of disease.

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