P-300 Effects of Nutrients on Miniguts Growth

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Abstract

Background:

The intestinal tract performs a duo role of absorbing substances for the body and protecting the body from the outside world. The intestinal epithelium is the part of the tract that absorbs nutrients through its large surface area. Since the intestinal epithelium is constantly bombarded with different chemicals and nutrients in performing its function, it is critical to determine what kind of nutrients enhance or inhibit intestinal health and functionalities. Miniguts derived from intestinal stem cells provide a new dimension to research and observe the way organs work. In this study, we conducted multiple tests of various nutrients on gut functionality using mice intestinal organoids as the model. Significant results observed were caffeic acid inhibited organoid growth in a concentration-dependent manner, curcumin exhibited a range of effectiveness, and vitamin C did not affect organoid growth.

Methods:

Because the major function of the intestinal tract is to absorb nutrients, liquids, and other substances for the body, it is important to understand the effects of ingesting different food nutrients on the gut. We utilized mice intestinal organoids to model a functional gut system. In this study, we aimed to determine the effects of the different food nutrients (MSG, vitamin C, chlorogenic acid, caffeic acid, curcumin, and mHPP) on gut function. The mice intestinal organoids were grown in 24 wells in 2 plates for 7 days without adding any nutrients. Pictures of the organoids were taken by the LAS in ×5 and ×10 magnification every other day. After growing for 7 days, food nutrients were added to the wells in 100, 300, and 600 μg/mL solutions. A group of organoids were not given any nutrients to be the control. After administering nutrients, pictures of the organoids were taken daily with the LAS software in ×5 and ×10 magnification. The surface areas of the organoids were calculated using ImageJ and graphed.

Results:

In this experiment, we observed the effects of various food nutrients. Curcumin exhibited a range around 300 μg/mL that stimulated organoid growth. This shows potential in cancer treatments and recoveries. Caffeic acid inhibited growth in higher concentrations, while vitamin C did not strongly affect organoid growth. mHPP inhibited growth slightly, but it is possible that it affects cells in other ways.

Conclusions:

Future work would be to find out how the nutrients affect the cells in ways that amplify or inhibit their growths. Other future work would be to observe the effects of MSG again because its results did not comply with previous studies. While chlorogenic acid exerted a slight influence on positive organoid growth in the 300 μg/mL concentration, there were not many significant impacts and should be observed again.

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