To investigate cyclin E, p16ink4a, and Ki67 as possible diagnostic biomarkers for cervical preneoplasia, and to evaluate its significance for screening human papillomavirus (HPV)-infected patients who are at high risk of developing cervical carcinoma.Methods:
The expressions of cyclin E, p16ink4a, and Ki67 were examined in 266 cervical exfoliated epithelial specimens using immunohistochemical analysis; simultaneously, HPV type 16/18 DNA status were detected by PCR.Results:
Overexpression of cyclin E was observed in low-grade squamous intraepithelial lesion (LSIL) (92.4% P < 0.005), and p16ink4a and Ki67 were overexpressed in high-grade squamous intraepithelial lesion (HSIL) (92.0% and 89.8%, respectively).Conclusions:
Cyclin E, p16ink4a, and Ki67 may be used as diagnostic biomarkers for HPV-related cervical neoplastic lesions. In addition, this technique can be used for screening patients at high risk of developing cervical carcinoma.