Cyclin E, p16ink4a, and Ki67 Overexpression as Possible Diagnostic Markers in HPV-Related Cervical Neoplasial Cell

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Abstract

Objectives:

To investigate cyclin E, p16ink4a, and Ki67 as possible diagnostic biomarkers for cervical preneoplasia, and to evaluate its significance for screening human papillomavirus (HPV)-infected patients who are at high risk of developing cervical carcinoma.

Methods:

The expressions of cyclin E, p16ink4a, and Ki67 were examined in 266 cervical exfoliated epithelial specimens using immunohistochemical analysis; simultaneously, HPV type 16/18 DNA status were detected by PCR.

Results:

Overexpression of cyclin E was observed in low-grade squamous intraepithelial lesion (LSIL) (92.4% P < 0.005), and p16ink4a and Ki67 were overexpressed in high-grade squamous intraepithelial lesion (HSIL) (92.0% and 89.8%, respectively).

Conclusions:

Cyclin E, p16ink4a, and Ki67 may be used as diagnostic biomarkers for HPV-related cervical neoplastic lesions. In addition, this technique can be used for screening patients at high risk of developing cervical carcinoma.

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