Fish orientation is consistently identified as a major influence on fish target strength (TS). Generally, orientation is defined as the tilt angle of the fish with respect to the acoustic transducer, whereas a more accurate definition includes tilt, roll, and yaw. Thus far, the influences of roll and yaw on fish TS have only been examined cursorily. We used in situ single-target data to create fish tracks, to estimate fish tilt and yaw, and correlated these estimates with TS. The results show that tilt, yaw, and beam position have a significant influence on fish TS. To investigate further how yaw and beam position affect TS, we calculated the expected backscatter from each fish within simulated fish aggregations using a backscatter model. The TS of individual fish at 38 and 120 kHz varied by as much as 11 and 19 dB with changes in yaw and beam position. Altering the fish’s tilt, yaw, and beam position resulted in TS differences of 14 and 26 dB at 38 and 120 kHz, respectively. Orientation had a minimal influence on an aggregation’s average TS if the aggregation had a variable tilt-angle distribution and was dispersed throughout the acoustic beam.