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The elastic-taping method of the proprioceptive neuromuscular facilitation (PNF) concept is nonexistent. Therefore, our study aimed to investigate the effect of treadmill training (TT) using PNF lower-leg taping (PNFLT) on walking and balance ability in patients with stroke. There were a total of 27 patients: a stroke allocated control group (n=13) and an experimental group (n=14). The control group performed the TT and the experimental group performed the TT using PNF taping five times a week for 6 weeks. Walking and balance ability were measured using the 6-min walk test (6MWT), the 10-m walking test (10MWT), and the timed up and go test (TUG). Before and after the intervention, a paired t-test was performed to compare different within-group differences. Independent t-tests were performed to compare different between-group differences. All statistical significance levels were set at α of 0.05. After intervention, 6MWT, 10MWT and TUG improved significantly in PNFLT-TT and TT group (P<0.01). After intervention, the PNFLT-TT group showed more effective changes that the TT group in 6MWT, 10MWT, and TUG (P<0.05 and <0.01) between PNFLT-TT and TT group. The intervention of PNFLT-TT was effective in improving walking and balance ability in patients with stroke. Basic elastic tape for the PNF concept and TT may be useful interventions as a program to improve walking and balance ability in patients with stroke.