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This in vitro study assessed the accuracy of multiple implant-level impression techniques (open tray and closed tray) for the fabrication of 3-unit implant prostheses with strain gauge (SG) analysis.A polyurethane master model was designed to simulate a clinical situation. Two rigid custom trays were fabricated for the model. The impression material used was polysulfide. Transfer implant impressions were made using 2 techniques; ten tapered copings not splinted (custom closed tray) and ten square copings splinted with acrylic-resin (custom open tray). The improved stone models were allowed to set for 1 hour before being separated from the impression. The superstructures were sprued, invested, and cast with a cobalt-chromium alloy. Four SGs were bonded on the surface of each polyurethane block.A statistically significant difference was found between the 2 impression techniques tested (P = 0.000). This study found a significant average difference of 144.68 ± 5.53 με for open custom tray and 253.3 ± 16.7 με for closed custom tray.The custom open tray technique was the most accurate impression for multiple implants compared with closed custom tray.