Colorectal cancer chemoprevention by 2 β-cyclodextrin inclusion compounds of auraptene and 4′-geranyloxyferulic acid


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Abstract

The inhibitory effects of novel prodrugs, inclusion complexes of 3-(4′-geranyloxy-3′-methoxyphenyl)-2-transpropenoic acid (GOFA) and auraptene (AUR) with β-cyclodextrin (CD), on colon carcinogenesis were investigated using an azoxymethane (AOM)/dextran sodium sulfate (DSS) model. Male CD-1 (ICR) mice initiated with a single intraperitoneal injection of AOM (10 mg/kg body weight) were promoted by the addition of 1.5% (w/v) DSS to their drinking water for 7 days. They were then given a basal diet containing 2 dose levels (100 and 500 ppm) of GOFA/β-CD or AUR/β-CD for 15 weeks. At Week 18, the development of colonic adenocarcinoma was significantly inhibited by feeding with GOFA/β-CD at dose levels of 100 ppm (63% reduction in multiplicity,p< 0.05) and 500 ppm (83% reduction in the multiplicity,p< 0.001), when compared with the AOM/DSS group (multiplicity: 3.36 ± 3.34). In addition, feeding with 100 and 500 ppm (p< 0.01) of AUR/β-CD suppressed the development of colonic adenocarcinomas. The dietary administration with GOFA/β-CD and AUR/β-CD inhibited colonic inflammation and also modulated proliferation, apoptosis and the expression of several proinflammatory cytokines, such as nuclear factor-kappaB, tumor necrosis factor-α, Stat3, NF-E2-related factor 2, interleukin (IL)-6 and IL-1β, which were induced in the adenocarcinomas. Our findings indicate that GOFA/β-CD and AUR/β-CD, especially GOFA/β-CD, are therefore able to inhibit colitis-related colon carcinogenesis by modulating inflammation, proliferation and the expression of proinflammatory cytokines in mice.

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