Kaempferol-3-o-β-d-glucuronate exhibit potential anti-inflammatory effect in LPS stimulated RAW 264.7 cells and mice model


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Abstract

Kaempferol-3-O-β-d-glucuronide (K3G) having various pharmacological effects was explored for its anti-inflammatory effect in LPS induced RAW 264.7 cells and mice model. K3G significantly inhibited various pro-inflammatory mediators like IL-1β, NO, PGE2, and LTB4. It upregulated the secretion of anti-inflammatory cytokine IL-10. K3G is found to reduce inflammation when studied for parameters like phagocytic index, carrageenan induced paw edema in mice and organ weight. It reduced inflammation in a dose dependent manner both in-vitro and in-vivo. Further molecular insights into the study reveal that K3G blocks the phosphorylation of NF-kB which is key regulator of inflammation, thereby exhibiting anti-inflammatory potential. Hence, this study permits further investigation to develop K3G as anti-inflammatory drug.Graphical abstractHighlightsK3G inhibited pro-inflammatory mediators IL-1β, NO, PGE2, LTB4 and upregulated anti-inflammatory cytokine IL-10.K3G reduced inflammation when studied for parameters like phagocytic index and carrageenan induced paw edema in mice.K3G exerts its effect by NF-kB suppressionAnti-inflammatory effect has been proved both in-vitro and in-vivo.All this data permit K3G to be established as an anti-inflammatory drug.

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