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A method of propagation through nodal culture was developed for Virginia round-leaf birch, Betula uber (Ashe) Fernald. Nodal cultures of Virginia round-leaf birch had the greatest shoot elongation on woody plant medium supplemented with 7.5 μM benzyladenine and 2.9 μM gibberellin-3. Shoot elongation was significantly greater when benzyladenine was combined with gibberellin-3 than with benzyladenine alone. In conjunction with propagation, anatomical changes in cuticle and stomata were examined at different stages of culture. Changes in stomatal length, stomatal density, cuticle thickness, and palisade parenchyma layers were noted during the various stages of culture. Propagation through nodal culture provides a feasible method by which Virginia round-leaf birch can be propagated and maintained. The same techniques may be applicable to other endangered hardwood species.