IgE and IgG anti–house dust mite specificities in allergic disease

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Abstract

Background

There are few studies that quantitatively compare IgE and IgG antibody binding to the major and minor house dust mite allergens.

Objective

To measure the IgE and IgG antibody specificities produced by adults and children, including children admitted to an emergency department for asthma.

Methods

Antibodies were measured by solid-phase microtiter assays.

Results

Children recruited from the emergency department had similar titers and patterns of IgE antibody binding compared with children without acute disease. Der p 1 and 2 bound 50% to 65% of the IgE antibody, and most of the remaining binding was to Der p 4, 5, and 7. Der p 3, 8, 10, and 20 induced low titers. The pattern was similar across a wide range of antihouse dust mite titers. IgG1 and IgG4 antibodies predominantly bound the major and midrange allergens and were mainly found in children with allergy. Children recruited in the emergency department had lower titers.

Conclusion

The same IgE antibody-binding pattern and predominant contribution of Der p 1 and 2 was found across a wide range of total IgE antibody titers and for children admitted to an emergency department. IgG1 and IgG4 antibodies bound to the more allergenic specificities and were largely found in children with allergy. The IgG antibody titers were lower in sera from children admitted to the emergency department for asthma exacerbations.

Clinical implications

Der p 1 and 2 and possibly Der p 4, 5, and 7 provide a formulation suitable for immunotherapy and diagnosis. Low IgG antibodies were a feature of acute disease.

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