The importance of studying risky behaviors in youth is determined by the initiation, development, and consolidation of different behaviors at this vital stage of life. Gender, as a cross-category analysis, has become one of the most decisive factors in the study of these behaviors. The aim of our study is to analyze the role of gender in risky behaviors (sexuality, road safety, and toxin consumption) of nursing students at the University of Seville. It is an analytical, observational, cross-sectional, and retrospective study. A self-administered questionnaire (N = 220) for first-year nursing students was used. Results indicate that gender is a variable that explains, from the unequal presence of men and women in pursuing nursing degrees, the different risky behaviors they adopt. The sample shows that risky behaviors among university students who are interested in health sciences are similar to those reported by younger people in the same and different context.