Nitroimidazole resistance genes (nimB) in anaerobic Gram-positive cocci (previously Peptostreptococcus spp.)

    loading  Checking for direct PDF access through Ovid

Abstract

Objectives

To investigate metronidazole resistance and the prevalence of nitroimidazole (nim) genes in clinically isolated anaerobic Gram-positive cocci.

Methods

Metronidazole susceptibility was determined in 99 strains of anaerobic Gram-positive cocci and PCR amplification for the nim gene carried out on 61 strains (metronidazole MIC ≥0.5 mg/L).

Results

The nimB gene was detected in 34% (21/61) of the strains. These included two highly resistant Finegoldia magna strains (MICs >128 mg/L). The nimB gene was, however, also demonstrated in 90% (19/21) of susceptible strains.

Conclusions

Although the nimB gene may be implicated in the high-level metronidazole resistance in 2 F. magna strains, the alarmingly high prevalence of the nimB gene in anaerobic Gram-positive cocci cannot be directly associated with resistance and the possibility of a silent nimB gene should be considered.

Related Topics

    loading  Loading Related Articles