Susceptibility of pseudomonads to Melaleuca alternifolia (tea tree) oil and components

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Abstract

Objectives

Thirty isolates of Pseudomonas aeruginosa, 15 isolates of Pseudomonas putida and 11 isolates of Pseudomonas fluorescens were tested for susceptibility to tea tree oil (TTO), the essential oil of Melaleuca alternifolia, and the components terpinen-4-ol, α-terpineol, cineole, γ-terpinene and ρ-cymene.

Methods

MICs were determined by broth microdilution in Mueller–Hinton medium supplemented with 0.002% (v/v) Tween 80.

Results

The MIC90 of TTO for all isolates tested was 4% (v/v) or less. Susceptibility to components tested varied between species.

Conclusions

Pseudomonas spp. are susceptible to TTO and some of its components although they are less susceptible than many other bacteria tested previously.

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