This experiment was conducted to investigate the effects of supplementation of probiotics and prebiotics on growth performance, nutrient digestibility, relative organ weight, fecal microbiota, blood profile, and excreta noxious gas emissions in broilers. A total of 714 one-day-old mixed sex ROSS 308 broilers with the initial BW = 40 ± 0.69 g was used in a 4-week trial. All birds were raised in wire cages. Birds were randomly allotted to 7 treatments with 6 replicates per treatment and 17 broilers per pen. Dietary treatments included: 1) T1 (control), 2) T2 [T1 + 0.2% probiotics (Bacillus sp. 1 × 109CFU, Lactobacillus sp. 1 × 108CFU, Aspergillus niger. 1 × 107CFU)], 3) T3 (T1 + 1% chicory fiber), 4) T4 (T1 + 1% rice bran), 5) T5 (T1+ 0.2% probiotics + 1% chicory fiber), 6) T6 (T1 + 0.2% probiotics + 1% rice bran), and 7) T7 (T1 + 0.2% probiotics + 1% chicory fiber + 1% rice bran). During d 1 to 14, broilers fed the T5, T6, and T7 diets had greater body weight gain (BWG) compared with the T1 diet (P < 0.05). Moreover, probiotics-included treatments resulted in higher BWG compared with the probiotics-free diets (P < 0.05). Broilers fed the T7 diet increased more in BWG compared with those fed the T1 diet, whereas feed conversion ratio (FCR) decreased more in the T7 diet than the T1 diet overall (P < 0.05). Dry matter (DM) digestibility increased more in the T5, T6, and T7 diets compared with the T1 diet (P < 0.05). Broilers fed probiotics-based diets had more improved DM digestibility compared with those fed probiotics-free diets (P < 0.05). Excreta Lactobacillus counts increased more in the T7 diet compared with the T1 diet (P < 0.05). Broilers fed with probiotics diets had higher excreta Lactobacillus counts compared with those fed the probiotics-free diets (P < 0.05). In conclusion, supplementation with 0.2% probiotics and 1% dietary fiber as prebiotics could improve the growth performance, DM digestibility, and excreta Lactobacillus counts in broilers raised in wire cages.