Centrifugal Pulsing Increases the Recovery of Viable Spermatozoa

    loading  Checking for direct PDF access through Ovid

Abstract

Purpose:

Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation.

Methods:

A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5°C in TEST-yolk results determined the efficacy of the centrifugation techniques.

Results:

The number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5°C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standard centrifugation.

Conclusions:

Pulsing the relative centrifugal field during centrifugation yields a higher number of viable spermatozoa.

Related Topics

    loading  Loading Related Articles