Isolation and Purification of an Early Pregnancy Factor-like Molecule from Culture Supernatants Obtained from Lymphocytes of Pregnant Women: II. Identification of the Molecule as a FC-Receptor-like Molecule: A Preliminary Report

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Early pregnancy factor (EPF)-like activity from culture supernatants obtained from stimulated lymphocytes of pregnant women was characterized and identified.


The enzyme-linked immunosorbent assay depending on the presence of "Fc" receptors on bovine spermatozoa was used to identify the EPF-like molecule purified by gel filtration and reverse-phase high-performance liquid chromatography.


The results indicated that the crude lymphocyte culture supernatant, the EPF-positive G IV fraction obtained on gel filtration, and the EPF-positive reverse-phase high-performance liquid chromatography protein readily bound with the different concentrations of aggregated human γ-globulin in a manner similar to that in which the standard control of aggregated human γ-globulin binds to the bovine spermatozoa.


EPF-like activity synthesized and secreted by lymphocytes during pregnancy may be a Fc-receptor-like molecule.

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