In Vivo Echocardiographic Imaging of Transplanted Human Adult Stem Cells in the Myocardium Labeled with Clinically Applicable CliniMACS Nanoparticles

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Abstract

Objectives

Intramyocardial transplantation of bone marrow–derived cells is currently under clinical evaluation as a therapy of heart failure. A major limitation of all clinical studies dealing with myocardial cell engraftment is the inability to track the fate of the transplanted cells. We present a clinically applicable technique using transesophageal echocardiography (TEE) of CliniMACS nanoparticle labeled transplanted CD133+ cells in ischemic hearts.

Methods and Results

CD133+ cells were isolated from human bone marrow by CliniMACS magnetic bead selection. Positive (5 × 106 cells) cells were transplanted into porcine ischemic myocardium (n = 6). Control animals (n = 5) received medium injection. TEE was performed to demonstrate the distribution of the cells during and 8 weeks after the procedure. Accumulations of labeled CD133+ cells of adjacent injections in the myocardium were discriminatively identified by TEE.

Conclusions

TEE is an elegant method for real-time documentation of successful transplantation and cell colony localization of magnetically labeled CD133+ cells in the ischemic myocardium. Our method should be of special interest for TEE-guided transcatheter injection of cells.

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