In Vivo Echocardiographic Imaging of Transplanted Human Adult Stem Cells in the Myocardium Labeled with Clinically Applicable CliniMACS Nanoparticles

    loading  Checking for direct PDF access through Ovid



Intramyocardial transplantation of bone marrow–derived cells is currently under clinical evaluation as a therapy of heart failure. A major limitation of all clinical studies dealing with myocardial cell engraftment is the inability to track the fate of the transplanted cells. We present a clinically applicable technique using transesophageal echocardiography (TEE) of CliniMACS nanoparticle labeled transplanted CD133+ cells in ischemic hearts.

Methods and Results

CD133+ cells were isolated from human bone marrow by CliniMACS magnetic bead selection. Positive (5 × 106 cells) cells were transplanted into porcine ischemic myocardium (n = 6). Control animals (n = 5) received medium injection. TEE was performed to demonstrate the distribution of the cells during and 8 weeks after the procedure. Accumulations of labeled CD133+ cells of adjacent injections in the myocardium were discriminatively identified by TEE.


TEE is an elegant method for real-time documentation of successful transplantation and cell colony localization of magnetically labeled CD133+ cells in the ischemic myocardium. Our method should be of special interest for TEE-guided transcatheter injection of cells.

Related Topics

    loading  Loading Related Articles