A New Approach for Rapidly Reshaping Single-Chain Antibody In Vitro by Combining DNA Shuffling with Ribosome Display

    loading  Checking for direct PDF access through Ovid


Antibody reshaping is an effective way to reduce the immunogenicity while maintaining or improving the affinity of murine antibodies. This paper describe a new in vitro approach for rapidly reshaping murine antibodies by combining DNA shuffling with ribosome display. With the new method, a reshaping anti–4-1BB single-chain antibody (scFv), Re-4B4-1 scFv, which bound to its antigen (4-1BB) specifically and strongly, was selected from a reshaping library. These results proved definitely the feasibility of the new designed approach for antibody reshaping.

Related Topics

    loading  Loading Related Articles