Mechanism of heat induction of albumin in early embryonic rat liver

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Abstract

Albumin is an adult liver specific protein whose induction in rats starts at day 19 or 20 of normal gestation. Our studies on the effect of heat stress on embryonic development showed premature induction of a 67 kDa protein at day 12 or 13 in embryonic liver cells, in addition to the induction of usual heat shock proteins. Immunoblotting with anti-albumin antibody confirmed the prematurely induced protein to be albumin. RNA dot blot showed that albumin induction upon heat shock is regulated at transcriptional level and northern blot confirmed the size of heat induced albumin transcript to be similar to the constitutively induced albumin RNA transcript.

During heat stress, heat shock proteins are induced by the interaction of a specific heat shock transcription factor (HSF) with specific DNA sequences (heat shock elements, HSEs) present in the promoters of all heat shock genes.

The functional significance of HSF-HSE interaction is confirmed by transient transfection assays using plasmids carrying chloramphenicol acetyl transferase reporter gene under the control of different deletion fragments of the rat albumin promoter. These assays identified the HSEs to be within -450 base pairs of the rat albumin promoter. Deletion of these HSE sequences from rat albumin promoter abolished its heat inducibility. Electrophoretic mobility shift assays with synthetic oligonucleotides, representing putative HSEs in the rat albumin promoter, and H4II-E-C3 cell extracts showed that the heat shock factor binds this region in a sequence specific and reversible manner. Gel super-shift assays with antibodies to HSF1 and HSF2 demonstrated that the HSEs present in the rat albumin promoter are bound by HSF1 but not by HSF2.

In addition to the HSEs, we have identified a putative GAGA factor binding site in the rat albumin promoter at -228 bp to -252 bp position. These GAGA repeats are bound in a sequence-specific and reversible manner by two factors in non-stressed cells, whereas only one of these two factors continues to bind the GAGA repeats under heat shock conditions.

We thus show that rat albumin promoter contains (i) functional HSEs to which the HSF1 binds and (ii) GAGA factor binding sites to which the GAGA factor binds and that the promoter activity can be modulated by temperature.

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