Background: Inflammatory bowel disease (IBD) includes Crohn's disease (CD) and ulcerative colitis (UC), chronic disease that still present challenges for physicians treating it: diagnosis, prognosis, and assessment. The current biomarkers for it are still limited. We aim to detect fecal miRNAs in IBD patients compare to healthy controls (HC), in order to get a novel and ideal biomarker for IBD.
Methods: Differential expression of fecal microRNAs micro-array for UC, CD and HC is analyzed, and validated by real-time polymerase chain reaction (RT-PCR).
Results: Seven miRNAs are selected by micro-array and literatures. RT-PCR shows that mir-16-5p is up-regulated in both UC and CD (p<0.01, p<0.01; respectively), while mir-21-5p is up-regulated just in UC (p=0.002). The sensitivity and specificity of mir-16-5p in UC are 83.3% and 88.2% (cut-off 10.92); The sensitivity and specificity of mir-16-5p in CD are 76.2% and 88.2%; The sensitivity and specificity of mir-21-5p in UC are 66.7% and 88.2% (cut-off 6.53). However, the sensitivity and specificity of erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) for UC and CD are lower than biomarkers detected above. For UC patients, mir-16-5p is correlated with age, disease duration, occult blood and S100A12 (p=0.02, r=0.56; p=0.02, r=0.53; p=0.02, r=0.54; p<0.01, r=0.75. respectively). For CD patients, mir-16-5p correlated none of the clinical factors.
Conclusions: The value of mir-16-5p and mir-21-5p in diagnosis of IBD are higher than ESR and CRP, they are not correlated with ESR and CRP, but correlated with occult blood, disease duration, albumin and platelet.