Background: The aim of this study is to compare two methods for measuring the concentration of faecal Calprotectin (FC) and assess the possibility of distinguishing between ulcerative colitis (UC) and irritable bowel syndrome (IBS).
Methods: Fifty-three patients with UC and 46 patients with IBS were prospectively included in the study. All patients were performed colonoscopy to confirm the diagnosis. Faecal calprotectin levels were analyzed by semiquantitative rapid test (CalDetect®) and an enzyme-linked immunosorbent assay (ELISA). Sensitivity and specificity of both assays were calculated.
Results: The sensitivity of the test set for direct semiquantitative directly to a value of 15μg/g was 78% and specificity of 83% for diagnosis of ulcerative colitis. For the ELISA to a value of 50 mg/g sensitivity and specificity were 83% and, respectively 93% (p=0.068).
Conclusions: Although the ELISA test has a higher diagnostic accuracy, it is not significantly higher compared to the semi-quantitative test directly CalDetect®). In addition, direct semi-quantitative test has the advantage of having immediate results is much easier to use in ambulatory patients.