Biological phantom with similar properties to the actual human breast is solution to dose estimation issues. It is definitely a safe environment for education and training purpose; therefore, the aim is always to attain the full potential of image quality. The main objective of this research was to develop a new quality-control biological phantom composed of readily available local and stable overtime material, inexpensive, and useful for mammography imaging modality. Many different local and unexpensive materials used in this research were tested using gas chromatography–mass spectrometry and were mixed by magnetic stirrer, and then HAFA phantom was designed and imaged using a mammography machine. Finally, optical density was measured by densitometer, which was close to human breast fat, and then, the materials were compared together.