Acute prolonged exercise has been shown to favorably alter lipid profiles in healthy, active adult males. To determine the effect of acute prolonged exercise in the individual with coronary heart disease (CHD), we measured total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in 12 healthy men (53.3 ± 11.8 years, JOURNAL/jcrh/04.02/00008483-199311000-00010/ENTITY_OV0335/v/2017-08-08T011949Z/r/image-png ± SD) and 11 men (64.2 ± 8.4 years) with documented CHD. Subjects performed a maximal exercise test and two submaximal exercise tests of 60 minutes each prescribed at 50% of maximal workload or 50% of heart rate reserve. Fasting blood samples were taken before, immediately following, and 24 hours after the submaximal exercise tests. Maximal oxygen uptake, maximal heart rate, and maximal workload achieved were significantly higher in the group without CHD. Blood lipid values changed minimally immediately post-exercise and 24 hours after exercise, between 0.64% to 5.3% and 0.53% to 8.6% respectively, and no changes reached statistical significance. Pretest triglyceride values were significantly related to the changes in triglyceride values both immediate post- and 24 hours post-exercise (r = .50-.53 respectively, P < 0.05). There were no statistically significant group differences nor any differences between the methods of exercise prescription. We conclude that a 60-minute exercise session at 50% of maximum, prescribed by workload or heart rate reserve, does not alter blood lipid concentrations; and there is no difference in the blood lipid response between patients with and without CHD.