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The present study aimed to assess canal preparation outcomes achieved by the new Reciproc Blue instrument using micro–computed tomography technology. M-Wire Reciproc was used as a reference instrument for comparison.Seven pair-matched mesial roots of mandibular molars presenting similar anatomic features of the canal (length, volume, surface area, and configuration) were selected after scanning procedures and assigned to 1 of the 2 groups according to the instrument used, M-Wire Reciproc and Reciproc Blue. After canal instrumentation, the specimens were rescanned, and the registered preoperative and postoperative datasets were examined to evaluate the percentages of removed dentin, untouched canal walls, and degree of canal transportation. Comparisons regarding the above outcomes between the 2 groups were done by using paired t test with the alpha-type set at 5%.Root canals prepared with conventional M-Wire Reciproc or Reciproc Blue were found to present similar shaping properties with no significant differences in the tested parameters.M-Wire Reciproc and Blue Reciproc presented similar shaping outcomes.Reciproc Blue instrument represents a new generation of reciprocating instruments.This study assessed the shaping ability of the new Reciproc Blue instrument through micro-CT technology.M-Wire Reciproc instrument was used as reference for comparison.Root canal preparation performed with both instruments presented similar shaping outcomes.