A 7.5-year-old male black-tailed prairie dog was presented for diarrhea of 3 weeks' duration. Clinical examination revealed a multinodular mass in the left cranial quadrant of the abdomen that was confirmed with ultrasound. An exploratory laparotomy was performed and the majority of the mass and adherent spleen were removed. Histological diagnosis was severe pancreatic nodular hyperplasia associated with secondary pancreatitis and passive splenic congestion. The prairie dog recovered without complications. Three weeks following the surgical procedure, follow-up ultrasound showed no evidence of abnormal tissue in the pancreaticoduodenal area. Two months postsurgery, a follow-up computed tomographic scan revealed no significant abnormalities. No recurrence was noted 13 months following the mass removal. This article describes the diagnosis of pancreatic nodular hyperplasia in a prairie dog and successful treatment of complications arising from the condition by partial pancreatectomy.